The contribution of biofilm to microbial growth rate in metalworking fluids
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چکیده
.................................................................................................................................................. 2 Abstract – Swedish .................................................................................................................................. 2 Swedish .................................................................................................................................. 2 Introduction ............................................................................................................................................. 3 Aim ...................................................................................................................................................... 6 Hypothesis ........................................................................................................................................... 7 Strategy ................................................................................................................................................ 7 Materials and methods ............................................................................................................................. 8 Downscale cultivation systems ........................................................................................................... 8 Methods for bacteria quantification................................................................................................... 12 Results ................................................................................................................................................... 17 Growth rate experiments ................................................................................................................... 17 Quantitative biofilm analysis ............................................................................................................. 26 Validation of biomass quantification methods .................................................................................. 28 Discussion ............................................................................................................................................. 32 Future work ........................................................................................................................................... 37 Acknowledgements ............................................................................................................................... 37 References ............................................................................................................................................. 37 Appendix ............................................................................................................................................... 41
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